stefan86's offtopic random retard thread - post away!

[PowerBlade]

Which one do i pick???

ADDITIONAL OLIGOS
start len tm gc% any 3' seq

1 LEFT PRIMER 401 20 59.99 45.00 7.00 2.00 TGAAAGCAATGCTGGAAGTG
RIGHT PRIMER 773 20 59.97 55.00 4.00 2.00 TACGCCCCGTTATAGGAGTG
PRODUCT SIZE: 373, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00

2 LEFT PRIMER 70 20 60.07 45.00 6.00 2.00 CATCTGCAGGACGGAAAAAT
RIGHT PRIMER 433 20 59.99 55.00 2.00 0.00 CGACTTCCTGCTTCACTTCC
PRODUCT SIZE: 364, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00

3 LEFT PRIMER 70 20 60.07 45.00 6.00 2.00 CATCTGCAGGACGGAAAAAT
RIGHT PRIMER 420 20 59.99 45.00 7.00 3.00 CACTTCCAGCATTGCTTTCA
PRODUCT SIZE: 351, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00

4 LEFT PRIMER 70 20 60.07 45.00 6.00 2.00 CATCTGCAGGACGGAAAAAT
RIGHT PRIMER 545 20 59.95 55.00 2.00 1.00 CCCACTACATCTGCCCCTAA
PRODUCT SIZE: 476, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

Statistics
con too in in no tm tm high high high
sid many tar excl bad GC too too any 3' poly end
ered Ns get reg GC% clamp low high compl compl X stab ok
Left 8247 0 0 0 15 0 880 5894 0 2 0 187 1269
Right 8316 0 0 0 9 0 743 6092 0 0 0 179 1293
Pair Stats:
considered 107, unacceptable product size 96, ok 11

4.

 

[gt_jen]

post
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[stefan86]

post-ah

 

[DarkGift]

fu

 

[DarkGift]

stefan, you're almost at the very top ov posters nau

 

[gt_jen]

i hate you

i feel so special

 

[stefan86]

we have to record primator 24, the necrophiler and chapter 666 before simon & garfunkel goes to pitt-eå!

 

[DarkGift]

be a man, man

 

[gt_jen]

stefan, you're almost at the very top ov posters nau

its true. but u cant beat my post rate

 

[DarkGift]

we have to record primator 24, the necrophiler and chapter 666 before simon & garfunkel goes to pitt-eå!

vimåstejude

 

[DarkGift]

its true. but u cant beat my post rate

orly

 

[DarkGift]

 

[DarkGift]

post

 

[bleak-]

lol retards

 

[gt_jen]

O yes

 

[gt_jen]

welcome you are now one of us


one of us



one of us

 

[stefan86]

oh, and don't forget mr. morbid fantasy. perfect EP

 

[vitorias123]

lol retards

you havent seen anything

 

[DarkGift]

lol retards

dukanvara

oh, and don't forget mr. morbid fantasy. perfect EP

yes, it was meant to become an epic EP

 

[stefan86]

you havent seen the worst of posting sprees yet